‘Smart’ cartilage cells programmed to release drugs when stressed

New technology could lead to treatments for osteoarthritis.

Researchers at Washington University School of Medicine in St. Louis have engineered cartilage cells to release an anti-inflammatory drug in response to stresses such cells undergo when they are compressed during weight bearing and movement. Here, the cell, called a chondrocyte, is stimulated with a very small glass pipette, about 1/5 the diameter of a human hair. When these cells undergo compression, they release the drug that combats inflammation. GUILAK LAB

by Jim Dryden, Washington University in St. Louis January 27, 2021

Working to develop new treatments for osteoarthritis, researchers at Washington University School of Medicine in St. Louis have genetically engineered cartilage to deliver an anti-inflammatory drug in response to activity similar to the bending of a knee or other motions that put stress on joints.

Among the early symptoms of osteoarthritis is pain in response to such movements — motions that involve the so-called mechanical loading of a joint. Joint pain that accompanies bending or lifting can make it difficult to perform normal activities. But by altering genes in cartilage cells in the laboratory, the researchers have been able to program them to respond to the mechanical stress associated with movement and weight-bearing by producing a drug to combat inflammation.

The study is published online Jan. 27 in the journal Science Advances.

“Drugs such as ibuprofen and naproxen that ease joint pain and lower systemic inflammation are the main treatments for osteoarthritis pain, but there are no therapies that actually prevent damage in the joints of patients with this debilitating form of arthritis,” said senior investigator Farshid Guilak, PhD, the Mildred B. Simon Professor of Orthopaedic Surgery. “We’ve developed a new field of research called mechanogenetics, where we can engineer cartilage cells to respond to the mechanical loading of the joint. Every time cells are under that stress, they produce an anti-inflammatory, biologic drug to reduce inflammation and limit arthritis-related damage.”

With his team, Guilak, a co-director of the Washington University Center of Regenerative Medicine and director of research at Shriners Hospitals for Children — St. Louis, first conducted experiments in the lab using cartilage cells from pigs to figure out how those cells sense when they are being mechanically stressed.

“Studying these cells in the lab, we were able to identify key pathways in the cells that respond to stress from loading and the gene circuits in cartilage that are activated by mechanical loading,” said co-first author Robert J. Nims, PhD, a postdoctoral researcher in Guilak’s laboratory.

Like the touch sensor on a smartphone, cartilage cells sense when stress is being applied, and the inflammation associated with the excessive stress of arthritis causes cartilage to break down. The cells developed in these experiments, however, responded to that stress by secreting an anti-inflammatory drug that blocked cartilage damage.

“We altered snippets of DNA in the cells to tell them to do something different than normal when they sense a load,” Guilak said. “That is, to make an arthritis-fighting drug.”

“It’s kind of like turning on a light,” said co-first author Lara Pferdehirt, a biomedical engineer and graduate research assistant in Guilak’s lab. “With a light, you flip a switch, and a lightbulb turns on. But in this case, the switch is the mechanical loading of a joint, and the bulb is the anti-inflammatory drug.”

The cells were engineered to release interleukin-1 receptor antagonist — a drug called anakinra (Kineret) that’s used to treat rheumatoid arthritis and shows promise for treating post-traumatic osteoarthritis that occurs following joint injury. Prior studies of the drug in patients with osteoarthritis have shown it to be safe but ineffective when only injected into a joint one time. Guilak believes that is because to work well, the drug must be released in arthritic joints over longer periods, while mechanical loading is occurring.

“This drug doesn’t seem to work unless it’s delivered continuously for years, which may be why it hasn’t worked well in clinical trials involving patients with osteoarthritis,” he said. “In our experiments in cells in the lab, we used existing signaling systems in the cartilage cells that we engineered so that they would release the drug whenever it’s needed. Here, we are using synthetic biology to create an artificial cell type that we can program to respond to what we want it to respond to.”

In addition to reducing inflammation in arthritic joints, having specific cartilage cells deliver the drug only when and where it’s needed should make it possible to avoid side effects associated with long-term delivery of a strong anti-inflammatory drug to the entire body. Those side effects can include stomach pain, diarrhea, fatigue and hair loss.

Guilak’s team plans to use the same technique to alter other types of cells to make different drugs.

“We can create cells that automatically produce pain-relieving drugs, anti-inflammatory drugs or growth factors to make cartilage regenerate,” Guilak said. “We think this strategy could be a framework for doing what we might need to do to program cells to deliver therapies in response to a variety of medical problems.”

This work was supported by Shriners Hospitals for Children and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases and the National Institute on Aging of the National Institutes of Health (NIH). Grant numbers AR76665, AG46927, AG15768, AR74240, AR73752, AR074992, AG28716. Additional support from the Nancy Taylor Foundation, the Arthritis Foundation, the National Science Foundation (an NSF EAGER Award and NSF Graduate Research Fellowship Program — DGE-1745038), The Phillip and Sima Needleman Fellowship, the Duke School of Medicine and a Duke Clinical and Translational Science Award.
Washington University School of Medicine’s 1,500 faculty physicians also are the medical staff of Barnes-Jewish and St. Louis Children’s hospitals. The School of Medicine is a leader in medical research, teaching and patient care, ranking among the top 10 medical schools in the nation by U.S. News & World Report. Through its affiliations with Barnes-Jewish and St. Louis Children’s hospitals, the School of Medicine is linked to BJC HealthCare.

Source Washington University in St. Louis 

 

  References

A mechanogenetic system was developed using a synthetic gene circuit to produce biologic drugs in response to mechanical loading, Robert J. Nims, Lara Pferdehirt, Noelani B. Ho, Alireza Savadipour, Jeremiah Lorentz, Sima Sohi, Jordan Kassab, Alison K. Ross, Christopher J. O’Conor, Wolfgang B. Liedtke, Bo Zhang, Amy L. Mcnulty, Farshid Guilak. Science Advances 27 Jan 2021 : Eabd9858 PDF

  Further reading

The role of macrophages in osteoarthritis and cartilage repair, Wu CL, Harasymowicz NS, Klimak MA, Collins KH, Guilak F. Osteoarthritis Cartilage. 2020 May;28(5):544-554. doi: 10.1016/j.joca.2019.12.007. Epub 2020 Jan 8.

An Emerging Target in the Battle against Osteoarthritis: Macrophage Polarization, Sun Y, Zuo Z, Kuang Y. Int J Mol Sci. 2020 Nov 12;21(22):8513. doi: 10.3390/ijms21228513. Full text

Designer Stem Cells: Genome Engineering and the Next Generation of Cell-Based Therapies, Guilak F, Pferdehirt L, Ross AK, Choi YR, Collins K, Nims RJ, Katz DB, Klimak M, Tabbaa S, Pham CTN. J Orthop Res. 2019 Jun;37(6):1287-1293. doi: 10.1002/jor.24304. Epub 2019 May 2. Full text

A Synthetic Gene Circuit for Self-Regulating Delivery of Biologic Drugs in Engineered Tissues, Pferdehirt L, Ross AK, Brunger JM, Guilak F. Tissue Eng Part A. 2019 May;25(9-10):809-820. doi: 10.1089/ten.TEA.2019.0027. Full text

Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs, Brunger JM, Zutshi A, Willard VP, Gersbach CA, Guilak F. Stem Cell Reports. 2017 May 9;8(5):1202-1213. doi: 10.1016/j.stemcr.2017.03.022. Epub 2017 Apr 27. Full text

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